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Human Immunodeficiency Virus 1 & 2 (HIV-1/HIV-2), Qualitative, RNA
CPT:
87535; 87538
Expected Turnaround Time
2 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Documents
For more information, please view the literature below.
Specimen Requirements
Specimen
Plasma or serum
Volume
1 mL
Minimum Volume
650 µL
Container
Lavender-top (EDTA) tube, BD SST tube, BD Vacutainer PPT plasma preparation tube
Collection
Collect specimen in lavender-top (EDTA), gel-barrier (SST tube) or a PPT plasma preparation tube. Specimens centrifugation within 24 hours of collection. Transfer serum or plasma to a polypropylene screw-cap tube for shipment.
Ship frozen.
Storage Instructions
Freeze (preferred) or refrigerate.
Stability Requirements
Temperature | Period |
|---|---|
Refrigerated | 7 days |
Frozen | 6 weeks |
Freeze/thaw cycles | Stable x3 |
Note: Stability provided by manufacturer or literature reference.
Causes for Rejection
Specimen grossly hemolyzed; specimen received at room temperature
Test Details
Use
This test is intended for use as an aid in the diagnosis of HIV-1 or HIV-2 infection, including acute or primary infection. Presence of HIV-1 or HIV-2 RNA in the plasma of patients without antibodies to HIV-1 or HIV-2 is indicative of acute or primary HIV-1 or HIV-2 infection. This assay may also be used as an additional test, when it is reactive, to confirm HIV-1 infection in an individual whose specimen is repeatedly reactive for HIV-1 and/or HIV-2 antibodies. This assay is not intended for use in donor testing.
Methodology
RT-PCR
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 139825 | HIV-1/HIV-2 Qualitative RNA | 96557-4 | 139826 | HIV-1 RNA | 25835-0 | |
| 139825 | HIV-1/HIV-2 Qualitative RNA | 96557-4 | 139827 | HIV-2 RNA | 69353-1 |
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CPT Statement/Profile Statement
The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf