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Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Nongraphical) With Reflex to HIV-1 GenoSure® MG
CPT:
87536
Synonyms
- HIV-1 Plasma Viremia
- HIV-1 RNA by Real-time PCR, Quantitative
- Reflex to Genotype
- Viral Load
Test Includes
Reflex to genotype (at additional charge) if viral load is >500 copies/mL
Special Instructions
This procedure does not provide serial monitoring; it is intended for one-time use only. If serial monitoring is required, please order test 550422.
Expected Turnaround Time
5 - 13 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Information
Related Documents
For more information, please view the literature below.
HIV-1 Lifecycle, Drugs, and Susceptibility Assays
Human Immunodeficiency Virus 1/O/2 Cascade: Fourth-generation Antigen/Antibody Detection
Specimen Requirements
Specimen
Plasma, frozen
Volume
6 mL
Minimum Volume
4 mL (Note: This volume does not allow for repeat testing.)
Container
Ship in a screw-cap polypropylene frozen transport tube.
Collection
Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Transfer plasma to one or more screw-cap polypropylene transport tube(s). "Snap-cap" or "pop-top" tubes should be avoided and may result in specimen rejection. Immediately freeze specimen at -20°C. Do not place samples on dry ice until completely frozen. Ship specimens frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Storage Instructions
Freeze. Stable at room temperature for one day or refrigerated for five days. Freeze for long-term storage.
Causes for Rejection
Hemolysis; green-top (heparin) tube; quantity not sufficient for analysis; frozen PPT™
Test Details
Use
Detect and quantitate HIV-1 in plasma with reflex to genotype (at additional charge) if viral load is >500 copies/mL. GenoSure® MG test detects mutations in the HIV viral genes that encode the HIV protease and polymerase (reverse transcriptase). Mutations in these genes have been associated with resistance to antiretroviral drugs.
Limitations
Assay range: 20−10,000,000 copies/mL
Methodology
Cobas® AmpliPrep/Cobas® TaqMan® HIV-1 Test, Version 2.0
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 550432 | RNA, PCR (NonGraph) rfx/Geno | 20447-9 | 551308 | HIV-1 RNA by PCR | copies/mL | 20447-9 |
| 550432 | RNA, PCR (NonGraph) rfx/Geno | 20447-9 | 551314 | log10 HIV-1 RNA | log10copy/mL | 29541-0 |
| 550432 | RNA, PCR (NonGraph) rfx/Geno | 20447-9 | 551272 | Genotype Assay | N/A |
| Reflex Table for Genotype Assay | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551568 | Reflex to GenoSure(R) MG | 551693 | 49573-9 | ||
| Reflex Table for Genotype Assay | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551568 | Reflex to GenoSure(R) MG | 551656 | HIV GenoSure(R) | 93047-9 | |
| Reflex 2 | 551318 | Quant, RNA PCR | 551311 | HIV-1 RNA by PCR | copies/mL | 20447-9 |
| Reflex Table for Genotype Assay | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551568 | Reflex to GenoSure(R) MG | 551656 | HIV GenoSure(R) | 93047-9 | |
| Reflex 2 | 551318 | Quant, RNA PCR | 551312 | log10 HIV-1 RNA | log10copy/mL | 29541-0 |
| Reflex Table for Genotype Assay | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551568 | Reflex to GenoSure(R) MG | 551656 | HIV GenoSure(R) | 93047-9 | |
| Reflex 2 | 551624 | HIV GenoSure(R) MG | 551624 | HIV GenoSure(R) MG | 49573-9 | |
| Reflex Table for Genotype Assay | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551568 | Reflex to GenoSure(R) MG | 551657 | HIV GenoSure(R) | 53798-5 | |
| Reflex 2 | 551619 | HIV GenoSure(R) MG Interp | 551619 | HIV GenoSure(R) MG Interp | 49573-9 | |
| Reflex Table for Genotype Assay | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551568 | Reflex to GenoSure(R) MG | 551566 | Tracking | N/A | |
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The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf