Human Immunodeficiency Virus 1 (HIV-1) Trofile® Select

TEST: 553355
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CPT: For coding, call Monogram Biosciences® at 800-777-0177.
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Synonyms

  • Coreceptor Tropism (Phenotypic)
  • HIV Coreceptor Tropism Phenotype
  • Tropism

Special Instructions

Both frozen plasma and frozen whole blood are required for this profile. If both samples are not received, the order for the assay will be cancelled.

Expected Turnaround Time

33 - 41 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

For more information, please view the literature below.

Coreceptor Tropism Assays

HIV Specimen Collection Guide

HIV-1 Lifecycle, Drugs, and Susceptibility Assays

    Specimen Requirements

    Specimen

    Plasma, frozen and whole blood, frozen

    Volume

    5 mL plasma and 4 mL whole blood

    Container

    Plasma: Lavender-top (EDTA); Whole blood: Lavender-top (EDTA)

    Collection

    Both frozen plasma and frozen whole blood are required for this profile. Draw four lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Plasma: Centrifuge three tubes within six hours of collection. Remove plasma, transfer plasma to screw-cap polypropylene transport tubes (not "pop-top" or "snap-cap"), and freeze. Ship specimen frozen. Whole blood: Do not centrifuge. Freeze tube immediately after draw. Ship specimen frozen. To avoid delays in turnaround time, when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

    Storage Instructions

    Freeze.

    Causes for Rejection

    Both frozen plasma and frozen whole blood not received; insufficient volume; heparinized plasma; specimen not frozen

    Test Details

    Use

    Trofile® Select allows for the determination of HIV-1 coreceptor tropism status, by which the appropriate assay, Trofile® or Trofile® DNA, is selected through an initial prescreening viral assessment. The coreceptor tropism status may be used to determine eligibility for CCR5 antagonist therapy, such as maraviroc (Selzentry®).

    Limitations

    This procedure should be used only for patients with documented HIV-1 infection.

    Methodology

    Polymerase chain reaction (PCR) amplification and viral culture. Samples are forwarded to Monogram Biosciences® for testing, reporting, and billing.

    References

    Whitcomb JM, Huang W, Fransen S, et al. Development and characterization of a novel single-cycle recombinant-virus assay to determine human immunodeficiency virus type 1 coreceptor tropism. Antimicrobial Agents Chemother. 2007 Feb; 51(2):566-575. 17116663

    LOINC® Map

    Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
    553355 Trofile(R) Select 553356 Trofile(R) Select N/A
    553355 Trofile(R) Select 829632 Trofile(R) PDF 51969-4
    553355 Trofile(R) Select 550341 Trofile(R) DNA PDF 51969-4
    Reflex Table for Trofile(R) Select
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 553101 Trofile(R) 829666 Tropotype: 50790-5
    Reflex Table for Trofile(R) Select
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 553101 Trofile(R) 829633 Trofile(R) 57182-8
    Reflex Table for Trofile(R) Select
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 553101 Trofile(R) 829634 Trofile(R) Interpretation 77202-0
    Reflex Table for Trofile(R) Select
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 553101 Trofile(R) 829635 Trofile(R) Methodology 49549-9
    Reflex Table for Trofile(R) Select
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 553101 Trofile(R) 829636 Trofile(R) References 75608-0
    Reflex Table for Trofile(R) Select
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 553102 Trofile(R) DNA 550347 Tropotype: 80690-1
    Reflex Table for Trofile(R) Select
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 553102 Trofile(R) DNA 829671 Trofile(R) DNA 50790-5
    Reflex Table for Trofile(R) Select
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 553102 Trofile(R) DNA 829672 Trofile(R) DNA Interpretation 62364-5

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