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Assessing infiltrating lymphoid activation state in human samples via a new 30-color spectral flow cytometry panel

November 6, 2024
SITC 2024 -- Flow cytometry is a common method to assess immune cell infiltration into tumors. Spectral flow cytometers are now being used to develop larger antibody panels to increase the number of immune markers analyzed. With a focus on immunotherapy and adoptive cell therapy, we have developed the 30-color Spectral CompLymphocyte™ Panel using the Cytek® Aurora Spectral Flow Cytometer. The panel backbone includes viability and 10 lineage markers to measure live T, NK and B cell subsets. Nineteen additional functional markers enable the characterization of activation, exhaustion, cytotoxicity and proliferation in each subset, plus memory T cell subset delineation.