Cross-Platform Validation for Paroxysmal Nocturnal Hemoglobinuria Diagnosis

AFC 2022 -- Paroxysmal nocturnal hemoglobinuria (PNH) is a rare and life-threatening blood disease characterized by populations of blood cells, called PNH clones, unable to express glycosylphosphatidyl-inositol (GPI)-linked proteins. The absence of these proteins such as CD55 or CD59, molecules known to be involved in the control of complement, render red blood cells (RBCs) susceptible to lysis by activated complement. Consequently, PNH patients often suffer from hemolytic anemia. For PNH diagnosis, flow cytometry is the preferred method to evaluate the presence of GPI-linked proteins on blood cells' surface with high sensitivity and specificity.