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Expected Turnaround Time
1 - 3 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Information
Related Documents
Specimen Requirements
Specimen
Urine
Volume
5 mL
Container
Sterile screw-cap container
Storage Instructions
Refrigerate.
Stability Requirements
| Temperature | Period |
| Refrigerated | Up to 14 days from collection (stability provided by manufacturer or literature reference) |
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Causes for Rejection
Swab specimen; inappropriate specimen transport device; unlabeled specimen or name discrepancy between specimen and request label; nonurine specimen
Test Details
Use
This test is used to confirm the diagnosis of Legionella pneumophila serogroup 1 infection.
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This test is used to confirm the diagnosis of Legionella pneumophila serogroup 1 infection. |
Limitations
This test will not detect species or serogroups other than Legionella pneumophila serogroup 1.
Methodology
Enzyme immunoassay (EIA)
Reference Interval
No Legionella pneumophila serogroup 1 antigen detected
Additional Information
Legionella pneumophila has been estimated to cause 80% to 85% of Legionella infections, with serogroup 1 accounting for most of these. Current methods of diagnosis for Legionella infection include culture and DFA. All of these other tests require a high quality respiratory specimen to perform this test and, unfortunately, many patients with Legionella infection do not produce sputum. A number of investigators have shown that a specific soluble antigen was present in the urine of patients with Legionnaires' disease, which can be detected both early and late in the disease.
References
Berdal PP, Farshy CE, Feelley JC. Detection of Legionella pneumophila antigen in urine by enzyme-linked immunospecific assay. J Clin Microbiol. 1979 May; 9(5):575-578. 383744
Kohler RB, Zimmerman SE, Wilson E, et al. Rapid immunoassay diagnosis of Legionnaires' disease: Detection and partial characterization of urinary antigen. Ann Intern Med. 1981 May; 94(5):601-605. 7235392
Reingold AL, Thomason BM, Brake BJ, Thacker L, Wilkinson HW, Kuritsky JN. Legionella pneumonia in the United States: The distribution of serogroups and species causing human illness. J Infect Dis. 1984 May; 149(5):819. 6726009
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