Acid-fast Organism Identification by PCR, MALDI-TOF and/or Sequencing

TEST: 183158
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CPT: 87150
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Synonyms

  • AFB Culture, Identification

Special Instructions

If reflex test is performed, additional charges/CPT code(s) may apply.

Expected Turnaround Time

15 - 24 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Specimen Requirements

Specimen

Isolated acid-fast bacillus (AFB) on agar or egg-based media; broth system positive for AFB (ensure it is not contaminated with non acid-fast bacteria)

Volume

Entire container

Container

Screw cap agar-slant tube; bacterial swab transport inoculated with isolated colonies from a pure culture; liquid-based broth tube from BD, bioMerieux or VersaTREK (slant, swab or liquid-based broth must be packed as an etiologic agent)

Storage Instructions

Maintain specimen at room temperature.

Causes for Rejection

Specimen received leaking or in broken transport tube or vial; unlabeled or name discrepancy between specimen and request label; primary clinical specimen received

Test Details

Use

This test is used to identify acid-fast bacteria referred from another laboratory that has performed the culture of the clinical sample. Note: PCR testing for identification of M. avium complex and M. tuberculosis complex will be performed on VersaTREK liquid-based broth submissions only. Organisms not identified by PCR will have additional charges for identification by MALDI-TOF and/or nucleic acid sequencing.

Limitations

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Methodology

Identification by PCR, MALDI-TOF and/or nucleic acid sequencing

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
183158 AFB Identification 183163 M tuberculosis complex 88874-3
183158 AFB Identification 183164 M avium complex 71719-9
183158 AFB Identification 183167 Reflex ID by MALDI N/A
183158 AFB Identification 183168 Other: N/A
Reflex Table for Reflex ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183402 Organism ID by MALDI 183403 Organism ID by MALDI 76346-6
Reflex 2 183404 Aerobic ID by MALDI 183404 Aerobic ID by MALDI 75756-7
Reflex 3 183285 Organism ID by Sequencing 183286 Organism ID by Sequencing 41852-5
Reflex 4 183269 Aerobic Bacteria, ID by Seq. 183270 Aerobic Bacteria, ID by Seq. 42803-7
Reflex 5 183271 Organism Identification 183272 Organism Identification 42803-7
Reflex Table for Reflex ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183402 Organism ID by MALDI 183403 Organism ID by MALDI 76346-6
Reflex 2 183404 Aerobic ID by MALDI 183404 Aerobic ID by MALDI 75756-7
Reflex 3 183285 Organism ID by Sequencing 183286 Organism ID by Sequencing 41852-5
Reflex 4 183301 Anaerobic Bacteria, ID by Seq. 183302 Anaerobic Bacteria, ID by Seq. 42803-7
Reflex 5 183303 Organism Identification 183304 Organism Identification 42803-7
Reflex Table for Reflex ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183402 Organism ID by MALDI 183403 Organism ID by MALDI 76346-6
Reflex 2 183404 Aerobic ID by MALDI 183404 Aerobic ID by MALDI 75756-7
Reflex 3 183285 Organism ID by Sequencing 183286 Organism ID by Sequencing 41852-5
Reflex 4 183277 Nocardia, ID by Sequencing 183278 Nocardia, ID by Sequencing 55096-2
Reflex 5 183279 Organism Identification 183280 Organism Identification 55096-2
Reflex Table for Reflex ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183402 Organism ID by MALDI 183403 Organism ID by MALDI 76346-6
Reflex 2 183404 Aerobic ID by MALDI 183404 Aerobic ID by MALDI 75756-7
Reflex 3 183285 Organism ID by Sequencing 183286 Organism ID by Sequencing 41852-5
Reflex 4 183293 Mycobacteria, ID by Seq. 183294 Mycobacteria, ID by Seq. 9825-1
Reflex 5 183295 Organism Identification 183296 Organism Identification 9825-1
Reflex Table for Reflex ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183402 Organism ID by MALDI 183403 Organism ID by MALDI 76346-6
Reflex 2 183404 Aerobic ID by MALDI 183404 Aerobic ID by MALDI 75756-7
Reflex 3 183285 Organism ID by Sequencing 183286 Organism ID by Sequencing 41852-5
Reflex 4 183319 Yeast, ID by Sequencing 183320 Yeast, ID by Sequencing 42804-5
Reflex 5 183321 Organism Identification 183322 Organism Identification 42804-5
Reflex Table for Reflex ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183402 Organism ID by MALDI 183403 Organism ID by MALDI 76346-6
Reflex 2 183404 Aerobic ID by MALDI 183404 Aerobic ID by MALDI 75756-7
Reflex 3 183285 Organism ID by Sequencing 183286 Organism ID by Sequencing 41852-5
Reflex 4 183327 Mold, ID by Sequencing 183328 Mold, ID by Sequencing 42804-5
Reflex 5 183329 Organism Identification 183330 Organism Identification 42804-5
Reflex Table for Reflex ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183402 Organism ID by MALDI 183403 Organism ID by MALDI 76346-6
Reflex 2 183405 Anaerobic ID by MALDI 183405 Anaerobic ID by MALDI 75756-7
Reflex 3 183285 Organism ID by Sequencing 183286 Organism ID by Sequencing 41852-5
Reflex 4 183269 Aerobic Bacteria, ID by Seq. 183270 Aerobic Bacteria, ID by Seq. 42803-7
Reflex 5 183271 Organism Identification 183272 Organism Identification 42803-7
Reflex Table for Reflex ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183402 Organism ID by MALDI 183403 Organism ID by MALDI 76346-6
Reflex 2 183406 Nocardia ID by MALDI 183406 Nocardia ID by MALDI 76346-6
Reflex 3 183285 Organism ID by Sequencing 183286 Organism ID by Sequencing 41852-5
Reflex 4 183269 Aerobic Bacteria, ID by Seq. 183270 Aerobic Bacteria, ID by Seq. 42803-7
Reflex 5 183271 Organism Identification 183272 Organism Identification 42803-7
Reflex Table for Reflex ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183402 Organism ID by MALDI 183403 Organism ID by MALDI 76346-6
Reflex 2 183407 Mycobacteria ID by MALDI 183407 Mycobacteria ID by MALDI 76346-6
Reflex 3 183285 Organism ID by Sequencing 183286 Organism ID by Sequencing 41852-5
Reflex 4 183269 Aerobic Bacteria, ID by Seq. 183270 Aerobic Bacteria, ID by Seq. 42803-7
Reflex 5 183271 Organism Identification 183272 Organism Identification 42803-7
Reflex Table for Reflex ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183402 Organism ID by MALDI 183403 Organism ID by MALDI 76346-6
Reflex 2 183408 Yeast ID by MALDI 183408 Yeast ID by MALDI 76346-6
Reflex 3 183285 Organism ID by Sequencing 183286 Organism ID by Sequencing 41852-5
Reflex 4 183269 Aerobic Bacteria, ID by Seq. 183270 Aerobic Bacteria, ID by Seq. 42803-7
Reflex 5 183271 Organism Identification 183272 Organism Identification 42803-7
Reflex Table for Reflex ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183402 Organism ID by MALDI 183403 Organism ID by MALDI 76346-6
Reflex 2 183428 Mold ID by MALDI 183428 Mold ID by MALDI 98404-7
Reflex 3 183327 Mold, ID by Sequencing 183328 Mold, ID by Sequencing 42804-5
Reflex 4 183329 Organism Identification 183330 Organism Identification 42804-5
Reflex Table for Reflex ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183402 Organism ID by MALDI 183403 Organism ID by MALDI 76346-6
Reflex 2 183327 Mold, ID by Sequencing 183328 Mold, ID by Sequencing 42804-5
Reflex 3 183329 Organism Identification 183330 Organism Identification 42804-5

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The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf